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Figure 2 PKCl/i deficiency influences activation of small GTPases. A: Protein lysates of deficient and control podocytes were used in activity assays for the small GTPases RhoA, <t>Rac1,</t> and Cdc42 and were analyzed using Western blot analysis. For loading control, the blotted membranes were incubated with GAPDH. B: Quantification of active GTPase versus total GTPase expression levels of RhoA, Rac1, and Cdc42. At least three different experiments of independent cell clones were used for each quantification. *P < 0.05, **P < 0.01.
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Figure 2 PKCl/i deficiency influences activation of small GTPases. A: Protein lysates of deficient and control podocytes were used in activity assays for the small GTPases RhoA, <t>Rac1,</t> and Cdc42 and were analyzed using Western blot analysis. For loading control, the blotted membranes were incubated with GAPDH. B: Quantification of active GTPase versus total GTPase expression levels of RhoA, Rac1, and Cdc42. At least three different experiments of independent cell clones were used for each quantification. *P < 0.05, **P < 0.01.
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Figure 2 PKCl/i deficiency influences activation of small GTPases. A: Protein lysates of deficient and control podocytes were used in activity assays for the small GTPases RhoA, <t>Rac1,</t> and Cdc42 and were analyzed using Western blot analysis. For loading control, the blotted membranes were incubated with GAPDH. B: Quantification of active GTPase versus total GTPase expression levels of RhoA, Rac1, and Cdc42. At least three different experiments of independent cell clones were used for each quantification. *P < 0.05, **P < 0.01.
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Figure 2 PKCl/i deficiency influences activation of small GTPases. A: Protein lysates of deficient and control podocytes were used in activity assays for the small GTPases RhoA, <t>Rac1,</t> and Cdc42 and were analyzed using Western blot analysis. For loading control, the blotted membranes were incubated with GAPDH. B: Quantification of active GTPase versus total GTPase expression levels of RhoA, Rac1, and Cdc42. At least three different experiments of independent cell clones were used for each quantification. *P < 0.05, **P < 0.01.
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Figure 2 PKCl/i deficiency influences activation of small GTPases. A: Protein lysates of deficient and control podocytes were used in activity assays for the small GTPases RhoA, <t>Rac1,</t> and Cdc42 and were analyzed using Western blot analysis. For loading control, the blotted membranes were incubated with GAPDH. B: Quantification of active GTPase versus total GTPase expression levels of RhoA, Rac1, and Cdc42. At least three different experiments of independent cell clones were used for each quantification. *P < 0.05, **P < 0.01.
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Figure 2 PKCl/i deficiency influences activation of small GTPases. A: Protein lysates of deficient and control podocytes were used in activity assays for the small GTPases RhoA, <t>Rac1,</t> and Cdc42 and were analyzed using Western blot analysis. For loading control, the blotted membranes were incubated with GAPDH. B: Quantification of active GTPase versus total GTPase expression levels of RhoA, Rac1, and Cdc42. At least three different experiments of independent cell clones were used for each quantification. *P < 0.05, **P < 0.01.
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Figure 2 PKCl/i deficiency influences activation of small GTPases. A: Protein lysates of deficient and control podocytes were used in activity assays for the small GTPases RhoA, <t>Rac1,</t> and Cdc42 and were analyzed using Western blot analysis. For loading control, the blotted membranes were incubated with GAPDH. B: Quantification of active GTPase versus total GTPase expression levels of RhoA, Rac1, and Cdc42. At least three different experiments of independent cell clones were used for each quantification. *P < 0.05, **P < 0.01.
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Image Search Results


Figure 2 PKCl/i deficiency influences activation of small GTPases. A: Protein lysates of deficient and control podocytes were used in activity assays for the small GTPases RhoA, Rac1, and Cdc42 and were analyzed using Western blot analysis. For loading control, the blotted membranes were incubated with GAPDH. B: Quantification of active GTPase versus total GTPase expression levels of RhoA, Rac1, and Cdc42. At least three different experiments of independent cell clones were used for each quantification. *P < 0.05, **P < 0.01.

Journal: The American journal of pathology

Article Title: Def-6, a novel regulator of small GTPases in podocytes, acts downstream of atypical protein kinase C (aPKC) λ/ι.

doi: 10.1016/j.ajpath.2013.08.026

Figure Lengend Snippet: Figure 2 PKCl/i deficiency influences activation of small GTPases. A: Protein lysates of deficient and control podocytes were used in activity assays for the small GTPases RhoA, Rac1, and Cdc42 and were analyzed using Western blot analysis. For loading control, the blotted membranes were incubated with GAPDH. B: Quantification of active GTPase versus total GTPase expression levels of RhoA, Rac1, and Cdc42. At least three different experiments of independent cell clones were used for each quantification. *P < 0.05, **P < 0.01.

Article Snippet: Activity Assays For activity measurements of the small GTPases, we used Rac1, Rho, and Cdc42 Activation Assay Biochem Kits (Cytoskeleton).

Techniques: Activation Assay, Control, Activity Assay, Western Blot, Incubation, Expressing, Clone Assay

Figure 7 Proposed model for influence of PKCl/i via Def-6 on the actin cytoskeleton. Deficiency of PKCl/i leads to increased activation of Def-6 and thereby to imbalance of the small GTPases, in particular Rac1. Active Rac1 influences several actin cytoskeletaledependent mechanisms.

Journal: The American journal of pathology

Article Title: Def-6, a novel regulator of small GTPases in podocytes, acts downstream of atypical protein kinase C (aPKC) λ/ι.

doi: 10.1016/j.ajpath.2013.08.026

Figure Lengend Snippet: Figure 7 Proposed model for influence of PKCl/i via Def-6 on the actin cytoskeleton. Deficiency of PKCl/i leads to increased activation of Def-6 and thereby to imbalance of the small GTPases, in particular Rac1. Active Rac1 influences several actin cytoskeletaledependent mechanisms.

Article Snippet: Activity Assays For activity measurements of the small GTPases, we used Rac1, Rho, and Cdc42 Activation Assay Biochem Kits (Cytoskeleton).

Techniques: Activation Assay